| 摘要 |
The invention provides novel compounds and compositions of Formulas I and II, as well as methods of using them. The compounds can be used, for example, to quantify an amount of double stranded DNA in a sample subjected to nucleic acid amplification, or for real time monitoring of a nucleic acid amplification reaction. The compounds can be provided in a kit, for example, with other reagents and instructions for using the compounds and reagents. |
| 主权项 |
1. A method determining cell viability, comprising:
a) contacting a sample comprising a cell with a compound according to Formula 1: wherein R1, R2 and R3 are each H Y is S or O; W taken together with the atoms to which it is attached is a 6-membered heterocyclic ring; n is O; Q is Q2: wherein Y is —CR13═CR14—; m is 1 and p is 0; R6 is unsubstituted (C1-C8)alkyl, unsubstituted aryl, or unsubstituted (C1-C8)alkaryl; R9, R11, and R12 are each independently H, amino, or halo; R10 is H, methoxy, amino or halo; one of R13 and R14 is H and the other is NR15R16; R15 is (C1-C6)alkyl or (C1-C6)alkyl-NR17R18 or C3H7—N+(CH3)3 wherein R17 and R18 are each independently H or (C1-C6)alkyl; and R16 is (C1-C6)alkyl-NR17R18 or C3H7—N+(CH3)3, wherein R17 and R18 are each independently H or (C1-C6)alkyl; wherein at least one of R15 and R16 is C3H7—N+(CH3)3; or a salt thereof; b) detecting a fluorescent signal in the sample; and c) correlating the amount of fluorescence in the sample with the viability of the cell in the sample. |
