CSN5 POLYPEPTIDES AND USES THEREOF FOR SCREENING THERAPEUTIC AGENTS

摘要

The present invention relates to mutated CSN5 polypeptides and their use in a method of screening modulators of CSN5 activity that could be used as therapeutic agents.

主权项

1. A polypeptide comprising
an amino acid sequence ranging from amino acid at position 53 to amino acid at position 252 in SEQ ID NO: 1 wherein the amino acid at position 106 in SEQ ID NO: 1 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 98 to amino acid at position 297 in SEQ ID NO: 2 wherein the amino acid at position 151 in SEQ ID NO: 2 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 58 to amino acid at position 257 in SEQ ID NO: 3 wherein the amino acid at position 111 in SEQ ID NO: 3 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 57 to amino acid at position 256 in SEQ ID NO: 4 wherein the amino acid at position 110 in SEQ ID NO: 4 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 55 to amino acid at position 254 in SEQ ID NO: 5 wherein the amino acid at position 108 in SEQ ID NO: 5 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 56 to amino acid at position 255 in SEQ ID NO: 6 wherein the amino acid at position 109 in SEQ ID NO: 6 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 61 to amino acid at position 260 in SEQ ID NO: 7 wherein the amino acid at position 114 in SEQ ID NO: 7 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 51 to amino acid at position 250 in SEQ ID NO: 8 wherein the amino acid at position 104 in SEQ ID NO: 8 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 52 to amino acid at position 251 in SEQ ID NO: 9 wherein the amino acid at position 105 in SEQ ID NO: 9 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 50 to amino acid at position 249 in SEQ ID NO: 10 wherein the amino acid at position 103 in SEQ ID NO: 10 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 50 to amino acid at position 249 in SEQ ID NO: 11 wherein the amino acid at position 103 in SEQ ID NO: 11 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 57 to amino acid at position 256 in SEQ ID NO: 12 wherein the amino acid at position 110 in SEQ ID NO: 12 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 57 to amino acid at position 256 in SEQ ID NO: 13 wherein the amino acid at position 110 in SEQ ID NO: 13 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 48 to amino acid at position 247 in SEQ ID NO: 14 wherein the amino acid at position 101 in SEQ ID NO: 14 is substituted by another amino acid, an amino acid sequence ranging from amino acid at position 56 to amino acid at position 255 in SEQ ID NO: 15 wherein the amino acid at position 109 in SEQ ID NO: 15 is substituted by another amino acid, or an amino acid sequence ranging from amino acid at position 75 to amino acid at position 273 in SEQ ID NO: 16 wherein the amino acid at position 127 in SEQ ID NO: 16 is substituted by another amino acid, and function-conservative variants thereof.