摘要 |
The embodiments of the present invention provide a method for the regeneration and differentiation of human perinephric fat derived mesenchymal stromal cells (hPNF-MSCs) into astroglial, renal, neuronal progenitor cells and pancreatic islet cells. The hPNF-MSCs are advantageous over bone marrow or other stem cell source due to the abundance and ease of isolation. The perinephric fat derived stromal cells are used in the treatment of neurodegenerative disorder, renal disorder and pancreatic malfunction. The method for the regeneration and differentiation of hPNF-MSCs includes isolation of the human perinephric fat derived stromal cells followed by expansion of the stem cells and cryopreservation. The cells are characterized by morphological evaluation of hPNF-MSC, growth kinetics, immunophenotypic evaluation, differentiation potential analysis, karyotyping and secretome profiling (cytokines, chemokines, and growth factors). The stem cells are differentiated into astroglial cells, neural cells renal cells and pancreatic islet cells. |