LYASE AND METHOD FOR ASYMMETRIC SYNTHESIS OF (S)-PHENYLACETYLCARBINOL

摘要

The invention relates to a lyase and to a method for asymmetric synthesis of (S)-phenylacetylcarbinol. According to the invention, a lysase is provided in which the isoleucine is substituted at position no. 468 in the protein ApPDC-E469G, which is modified in comparison to the wild type protein from Aceobacter pasteurianus, with an amino acid that occupies less space than isoleucine. In an advantageous embodiment, the amino acid tryptophan in position no. 543 of the enzyme according to the invention is replaced by a different amino acid that occupies less space than tryptophan. The enzymes according to the invention can be used for asymmetric synthesis of (S)-phenylacetylcarbinol. Enantiomeric excesses of up to 98 % ee can thereby be achieved.