MICROFLUIDIC SYSTEMS AND METHODS FOR HYDRODYNAMIC MICROVORTICAL CELL ROTATION IN LIVE-CELL COMPUTED TOMOGRAPHY

摘要

Microfluidic devices for 3D hydrodynamic microvortical rotation of at least one live single cell or cell cluster, systems incorporating the devices, and methods of fabricating and using the devices and systems, are provided. A microfluidic chip rotates at least one live single cell or cell cluster in a microvortex about a stable rotation axis perpendicular to an optical axis within a chamber having a trapezoidal cross-sectional shape located below a flow channel. An optical trap may be used to position the cell or cells with the microvortex, and the cell or cells may be subject to live-cell or cell cluster computer tomography imaging.

主权项

1. A device for 3D hydrodynamic microvortical rotation of at least one live single cell or cell cluster, the device comprising a microfluidic flow channel and at least one microfluidic chamber arranged below the microfluidic flow channel, wherein:
the at least one microfluidic chamber comprises an inlet opening arranged along a lower boundary of the microfluidic flow channel, wherein the inlet opening permits fluid communication between the at least one microfluidic chamber and the microfluidic flow channel; and the at least one microfluidic chamber comprises a trapezoidal cross-sectional shape transverse to a primary direction of flow in the microfluidic flow channel.