EXPANDED RADIX FOR POLYMERIC TAGS

摘要

A method having steps of (a) providing nucleic acids having a tag sequence (N1)n(N2)n . . . (Nx)n, wherein N1, N2 and Nx are nucleotides that complement different nucleotides, respectively, wherein n is an integer that can differ for N1, N2 and Nx; (b) detecting the nucleic acids individually and under conditions to distinguish signal intensities for (N1)n sequences having different values for n, (N2)n sequences having different values for n and. (Nx)n sequences having different values for n; and (c) distinguishing the tags based on the signal intensities.

主权项

1. A method, comprising
(a) providing a plurality of nucleic acid molecules, comprising individual nucleic acid types having a tag sequence,
wherein the tag sequence comprises the sequence (N1)n(N2)n,wherein N1 and N2 are nucleotides that complement different nucleotides, respectively,wherein n is a non-zero integer that can differ for N1 and N2,wherein the plurality of nucleic acid molecules comprises at least 48 of the tag sequences that are not the same; (b) detecting the individual nucleic acids under conditions to distinguish signal intensities for (N1)n sequences having different values for n and to distinguish signal intensities for (N2)n sequences having different values for n; and (c) distinguishing the nucleic acid tags based on the signal intensities detected in step (b).