| 主权项 |
1. A method for producing a recombinant antibody in a host cell comprising:
a. providing a genetically modified cell that has been genetically engineered to produce an antibody having sialylated N-glycans, wherein the host cell comprises a nucleic acid encoding the heavy chain of the antibody, and wherein the nucleic acid encodes mutations at amino acid positions 243 and 264 of the heavy chain, wherein the mutations at positions 243 are selected from the group consisting of: F243A, F243G, F243S, F243T, F243V, F243L, and F243I and the mutations at position 264 are selected from the group consisting of: V264A, V264G, V264S and V264T; b. culturing the transformed host cell under conditions which induce expression of the antibody; and c. isolating the antibody from the transformed host cell to produce the recombinant antibody having sialylated N-glycans. |
