发明名称 |
LYASE AND LYASE-ENCODING DNA, VECTORS CONTAINING THE DNA, AND METHOD FOR THE ASYMMETRIC SYNTHESIS OF (S)-PHENYLACETYLCARBINOL |
摘要 |
The invention relates to a lyase and a lyase-encoding DNA, to vectors containing the DNA and to a method for the asymmetric synthesis of (S)-phenylacetylcarbinol. According to the invention, a lyase is provided, in which tryptophan is replaced with an amino acid at position 543 in protein ApPDC-E469G, said protein being modified with respect to the wild type of Aceobacter pasteurianus, or in which it is less space-filling than tryptophan. According to the invention, deoxyribonucleic acids are furthermore provided, which encode the lyase. (S)-phenylacetylcarbinol can be produced with the lyase according to the invention from the educts benzaldehyde and pyruvate or acetaldehyde with an enantiomeric excess of at least 94 %. |
申请公布号 |
WO2016041533(A1) |
申请公布日期 |
2016.03.24 |
申请号 |
WO2015DE00407 |
申请日期 |
2015.08.14 |
申请人 |
FORSCHUNGSZENTRUM JÜLICH GMBH |
发明人 |
ROTHER, DÖRTE;POHL, MARTINA;SEHL, TORSTEN;MARX, LISA;WESTPHAL, ROBERT |
分类号 |
C12N9/88 |
主分类号 |
C12N9/88 |
代理机构 |
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