| 摘要 |
A dedicated device for preparing a tissue and an organ by using a spin accumulation method comprises a supporting apparatus, a multi-nozzle assembly, a motion mechanism, a shaping apparatus, and a control system. In the method for preparing a tissue and an organ by using a spin accumulation method, based on a core dissolving technology and a cell assembly technology, various cell growth factors, inhibitors or medicines are compounded into a cross-linkable/polymerized hydrogel material; filamentous hydrogel extruded from nozzles is crosslinked/polymerized and compositely synthesized into a polymer solution, is washed by a culture solution and then is wound and accumulated on a rotary inner core; the hydrogel containing various types of seed cells and the hydrogel to which endothelial cells and nerve cells are adhered separately form a main body part, a blood vessel and a nerve system; and the inner core is dissolved after completion of forming and the tissue and organ with spiral blood vessels and nerves are obtained after being cultured for a period of time. The method is simple and is easy to form a blood vessel and a nerve system, and is especially suitable for forming a tissue and organ with a lumen that is difficult to be formed by using existing tissue engineering. |
