SYSTEM AND METHOD OF PRECONCENTRATING ANALYTES IN A MICROFLUIDIC DEVICE

摘要

A method and system for preconcentrating analytes at a microvalve in a microfluidic device is disclosed. The system includes a sample channel loaded with a sample solution. The sample channel includes a semi-permeable membrane microvalve. An electric potential is applied at or across the microvalve to preconcentrate the sample solution when the microvalve is closed. The method includes pretreatments of the device or valve for preconcentration of the analytes. For preconcentration of anionic analytes, the device is baked. For preconcentration of the cationic analytes, the surface of the membrane microvalve is coated with a polycationic coating, and the device is baked.

主权项

1. A method of preconcentrating analytes at a microvalve in a microfluidic device comprising:
a. loading a sample solution into a sample channel, wherein the sample channel includes a semi-permeable membrane microvalve; b. applying an electric voltage at or across the microvalve to preconcentrate the sample solution when the microvalve is closed; c. introducing the sample solution to a separation channel when the microvalve is opened, wherein the separation channel is coupled to a capillary holder; and d. positioning a tapered or etched end of a capillary into the separation channel such that the interface between the capillary and the separation channel is free of dead volume.