发明名称 |
A method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides |
摘要 |
Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase- mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo -bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker. |
申请公布号 |
AU2014306271(A1) |
申请公布日期 |
2016.03.24 |
申请号 |
AU20140306271 |
申请日期 |
2014.08.08 |
申请人 |
THE SCRIPPS RESEARCH INSTITUTE |
发明人 |
ROMESBERG, FLOYD E.;MALYSHEV, DENIS A.;LI, LINGJUN;LAVERGNE, THOMAS;LI, ZHENGTAO |
分类号 |
C07H19/00;C12N15/09 |
主分类号 |
C07H19/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|