| 摘要 |
PROBLEM TO BE SOLVED: To enable biological organs, tissues, and cells to be applied for reconstructive surgery or the like by preserving them under the electric field space where the state which causes supercooling is ready, the supercooling causing the nonfreezing state of water at a temperature of the freezing point or less.SOLUTION: The invention relates to a preservation method for biological tissues or cells. The method comprises mounting the tissues or cells under the electric field space where the temperature is controlled at from -4°C to 4°C, the state which causes supercooling is ready, the supercooling causing the nonfreezing state of water at a temperature of the freezing point or less, the electric field strength is 0.3-1.0 kV/m; enabling the biological tissues or cells to be preserved for 60 to 84 hours without causing a morphological change; and forming the electric field space in the range within a radius of 50 cm centering on the electrode by applying the AC voltage of 1.0-3.5 kV to the electrode to make endothelial cells from the biological tissues or cells.SELECTED DRAWING: Figure 14 |
