PURIFICATION OF RECOMBINANTLY PRODUCED POLYPEPTIDES
摘要
Described herein is a method for separating a recombinantly produced polypeptide from host cell protein. The method includes a step of loading a clarified cell culture supernatant that includes the recombinantly produced polypeptide and the HCP onto a Protein A chromatography column and washing the Protein A chromatography column with a wash buffer comprising a fatty acid having a chain length of at least about 6 carbon atoms, or a fatty acid salt thereof to remove HCP and then recovering the recombinantly produced polypeptide.
申请公布号
EP2997036(A1)
申请公布日期
2016.03.23
申请号
EP20140797310
申请日期
2014.05.13
申请人
MEDIMMUNE LIMITED;MEDIMMUNE, LLC
发明人
GRUBER, DAVID, EDWIN;TURNER, RICHARD, EDWARD;BEE, JARED, SAMUEL;AFDAHL, CHRISTOPHER, DOUGLAS;TIE, LIU