发明名称 |
Procedimiento para purificar vectores víricos que tienen proteínas que se unen a ácido siálico |
摘要 |
A method for isolating, from a mixture, a virus having a surface protein with a binding site for sialic acid is provided. The method involves contacting the mixture with mucin which has been linked to a solid support and washing the solid support to remove material from the mixture is non-specifically bound to the mucin-linked support. Thereafter, the specifically bound virus (e.g., AAV4 or AAV5) may be removed in a further washing step utilizing a concentrated slat or solution with low pH. Also described are pharmaceutical kits containing solid supports linked to mucin for use in isolating virus having a surface protein with a binding site for sialic acid, or detecting the presence of the virus in a biological sample. |
申请公布号 |
ES2564553(T3) |
申请公布日期 |
2016.03.23 |
申请号 |
ES20020786355T |
申请日期 |
2002.08.06 |
申请人 |
The Trustees of the University of Pennsylvania |
发明人 |
WILSON, James M.;AURICCHIO, Alberto;HILDINGER, Markus |
分类号 |
C07K14/005;C12N15/09;C07K1/22;C07K14/015;C07K14/075;C12N7/00;C12N7/01;C12N7/02;C12N15/86;C12N15/864 |
主分类号 |
C07K14/005 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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