发明名称 |
Method for measuring calcineurin activity |
摘要 |
The present invention relates to a method for measuring the activity of calcineurin in a biological sample wherein a kinase inhibitor is present in the assay reaction mix. |
申请公布号 |
US9290792(B2) |
申请公布日期 |
2016.03.22 |
申请号 |
US201314381533 |
申请日期 |
2013.02.14 |
申请人 |
UNIVERSITE PARIS DESCARTES;ASSISTANCE PUBLIQUE HOPITAUX DE PARIS |
发明人 |
Sanquer Sylvia;Barouki Robert;Herry Laurence |
分类号 |
C12Q1/42 |
主分类号 |
C12Q1/42 |
代理机构 |
Marshall, Gerstein & Borun LLP |
代理人 |
Marshall, Gerstein & Borun LLP |
主权项 |
1. A method for measuring the activity of calcineurin in a biological sample comprising the steps of:
providing a reaction mix comprising a substrate, at least one phosphatase inhibitor and at least one kinase inhibitor; incubating said biological sample with the reaction mix under conditions suitable for the activity of calcineurin; measuring the amount of dephosphorylated substrate. |
地址 |
Paris FR |