| 主权项 |
1. A single-protein nanocapsule comprising a single-protein core and an artificial membrane covalently anchored to and encapsulating said single-protein, said artificial membrane comprising:
(a) a polymer network comprising repeated units of at least two different monomer units which independently are positively charged, negatively charged, or uncharged, and hydrophobic or hydrophilic, wherein: said polymer network is crosslinked by a crosslinker comprising two or more polymerizable groups capable of forming a crosslinked co-polymer between the single protein core and at least one monomer unit; and said crosslinker is selected from the group consisting of N,N′-bis(acryloyl)cystamine, bis[2-(methacryloyloxy)ethyl]phosphate, and bisacryloylated polypeptide, and is disposed in the nanocapsule so as to be degradable in vivo; (b) a surface charge at physiological pH which is positive charge, negative charge or no charge; and (c) a modifiable moiety, wherein said single protein is more resistant to protease. |
