Electroblot transfer buffer

摘要

A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.

主权项

1. An electroblot transfer method comprising
assembling a membrane and a gel, the gel containing at least one of separated proteins, peptides, or nucleic acids, the membrane and the gel sandwiched between at least two pieces of absorbent material, the absorbent material saturated with a transfer buffer providing a source of ions and comprising N-(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine (tricine), tris(hydroxymethyl)aminomethane (tris), glycine, and optionally ethylenediaminetetraacetic acid (EDTA); and applying a current to effect reduced resistance in transfer of at least one of the separated proteins, polypeptides, or nucleic acids from the gel to the membrane, wherein said ions of said saturated absorbent material drive said transfer.