Glucoamylase variants with altered properties

摘要

The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

主权项

1. A polynucleotide encoding a glucoamylase variant comprising amino acid substitutions at the positions corresponding to positions 417, 430, and 563 of SEQ ID NO: 2, or equivalent positions in a parent glucoamylase having at least 90% sequence identity to SEQ ID NO: 1 or 2, and
wherein the glucoamylase variant has at least 90% sequence identity with SEQ ID NO: 1 or 2 and glucoamylase activity.