Biomarkers for determining an allograft tolerant phenotype

摘要

Methods are provided for determining whether a subject has a graft tolerant phenotype. In practicing the subject methods, the expression level of one or more gene in a sample from the subject, e.g., a blood sample, is assayed to obtain a gene expression result, where the gene expression result includes a result for a biomarker of graft tolerance. The obtained gene expression result is then employed to determine whether the subject has a graft tolerant phenotype. Also provided are compositions, systems and kits that find use in practicing the subject methods. The methods and compositions find use in a variety of applications, including the determination of an immunosuppressive therapy regimen.

主权项

1. A method of determining whether a subject who has received renal allograft has a graft tolerant or a graft intolerant phenotype comprising:
(a) evaluating the level of expression of at least three genes in a sample from a subject who has received a renal allograft to obtain a gene expression result, wherein said at least three genes are selected from the group consisting of: BNC2, C1QC, CCL4, CYP1B1, FAM110C, GDEP, IGFL2, IGH@, IGHA2, IGHG4, IGJ, KLF6, NXF3, PRAMEF3, RLBP1L1, SHCBP1, SPC25, TFDP3, TNFRSF17, UHRF1 and VN1R2, wherein said evaluating comprises: contacting said sample with a collection of reagents for assaying for an expression product of each of said at least three genes; and assessing, using said collection of reagents, the amount of each of said expression products in said sample; (b) comparing said gene expression result to at least one reference gene expression profile, wherein said reference gene expression profile is selected from: a graft tolerant phenotype gene expression profile and a graft intolerant phenotype gene expression profile; and (c) determining that said subject has a graft tolerant phenotype based on said comparing when said gene expression result is similar to said graft tolerant phenotype gene expression profile and/or is dissimilar to said graft intolerant phenotype gene expression profile; or determining that said subject has a graft intolerant phenotype based on said comparing when said gene expression result is dissimilar to said graft tolerant phenotype gene expression profile and/or is similar to said graft intolerant phenotype gene expression profile.