| 发明名称 | Chromatography ligand comprising domain C from <i>Staphylococcus aureus </i>protein A for antibody isolation | ||
| 摘要 | The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali. | ||
| 申请公布号 | US9290549(B2) | 申请公布日期 | 2016.03.22 |
| 申请号 | US201414164519 | 申请日期 | 2014.01.27 |
| 申请人 | GE Healthcare Bio-Sciences AB | 发明人 | Hall Martin;Larsson Sture;Muranyi Andreas;Rodrigo Gustav;Zou Jinyu;Aberg Per-Mikael |
| 分类号 | C07K17/00;C07K14/31;B01D15/38;B01J20/286;C07K1/22;B01J20/32;C07K16/00 | 主分类号 | C07K17/00 |
| 代理机构 | Parks Wood LLC | 代理人 | Parks Wood LLC |
| 主权项 | 1. A chromatography ligand, which ligand comprises one or more Domain C units from Staphylococcus protein A (SpA), wherein the Domain C sequence includes the amino acid sequence as defined by SEQ ID NO 1, with the mutation G29A or the Domain C sequence includes the amino acid sequence as defined by SEQ ID NO 2, with the mutation G25A. | ||
| 地址 | Uppsala SE | ||