Methods for culture of microorganisms

摘要

Methods for cultivating photosynthetic microorganisms are described. The method includes the steps of injecting a liquid culture medium into a photosynthetic reactor according to a controlled flow rate, injecting a gas into the reactor according to a controlled flow rate through a gas injector of the reactor so that the gas injected through the gas injector moves up to as far as the exhaust by circulating in the reaction pipe, in a direction of circulation from the low end to as far as the high end of the reaction pipe, circulating the liquid culture medium with the circulation device, and controlling the circulation device and the gas injector in order to establish in at least one substantially horizontal reaction section of the reaction pipe gas/liquid two-phase flow conditions of the laminated flow or slug flow or elongated bubble flow type.

主权项

1. A method of cultivating photosynthetic microorganisms comprising:
using a photosynthetic reactor comprising
at least one photosynthetic reaction pipe in which the liquid culture medium circulates and which is provided with at least one substantially horizontal reaction section, said reaction pipe having a low end positioned in the low portion of the reactor and a high end positioned in the high portion of the reactor above the low end;at least one return pipe ensuring the fluidic connection between the low end and the high end of the reaction pipe;at least one circulation device for circulating the liquid culture medium;at least one gas injector positioned in the reaction section or upstream from said reaction section with respect to the direction of the circulation of the gas, said gas injector allowing gas to be injected into the reactor; andat least one exhaust positioned in the high portion of the reactor and allowing the gas injected into the reactor to escape, injecting a liquid culture medium into the reactor according to a controlled flow rate; injecting a gas into the reactor according to a controlled flow rate through the gas injector so that the gas injected through the gas injector moves up to as far as the exhaust by circulating in the reaction pipe, in a direction of circulation from the low end to as far as the high end of the reaction pipe; circulating the liquid culture medium with the circulation device; controlling the circulation device and the gas injector in order to establish in at least one substantially horizontal reaction section of the reaction pipe, gas/liquid two-phase flow conditions of the laminated flow or slug flow or elongated bubble flow type.