发明名称 |
Methods and compositions for separating rare cells from fluid samples |
摘要 |
The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components. |
申请公布号 |
US9290812(B2) |
申请公布日期 |
2016.03.22 |
申请号 |
US201414289444 |
申请日期 |
2014.05.28 |
申请人 |
Aviva Biosciences Corporation |
发明人 |
Yamanishi Douglas T.;Hujsak Paul G.;Snyder Sara F.;Walker George;Xu Junquan;Huang Mingxian;Tao Guoliang;Wu Lei;Wang Xiaobo;Ouyang Joe;Schmitigal Charina;Cheng Jing;Xu Jia |
分类号 |
C12Q1/68;G01N1/40;G01N33/569;G01N33/574;C12N5/071;B01L3/00 |
主分类号 |
C12Q1/68 |
代理机构 |
Morrison & Foerster LLP |
代理人 |
Morrison & Foerster LLP |
主权项 |
1. A method of enriching rare cells from a body fluid sample, comprising:
a) centrifuging a body fluid sample to obtain a sample wash pellet and a supernatant; b) resuspending said sample wash pellet to obtain a washed sample for rare cell enrichment; c) performing at least one debulking step on said washed sample for rare cell enrichment; and d) performing at least one separation step on said washed sample for rare cell enrichment to obtain enriched rare cells, wherein said separation step comprises adding to said washed sample at least one antibody or fragment thereof that selectively binds an undesirable component in said washed sample. |
地址 |
San Diego CA US |