Method for measuring cells, and reagent for cell measurement

摘要

A method and a means are provided, by which multiple types of cells can be simultaneously measured with high sensitivity by an ATP luminescence method. A method for measuring cells in a sample is provided, which comprises the steps of adding methanol to a sample suspected of containing viable cells to increase ATP within viable cells, extracting intracellular ATP, and causing extracted ATP to emit luminescence.

主权项

1. A method for measuring cells in a sample, comprising:
a first step comprising adding a solution comprising 0.1% to 20% methanol to a sample suspected of containing viable cells to increase ATP within the viable cells and adding an enzyme to remove ATP outside of the viable cells: a second step of extracting intracellular ATP following the first step of increasing intracellular ATP and removing extracellular ATP; and a third step of causing the extracted intracellular ATP to emit luminescence and measuring a luminescence level thereof.