| 摘要 |
Objects of the invention are to provide modified carbonyl reductases having improved reactivity in the presence of halogen atoms in comparison with a wild-type enzyme, as well as a method for efficiently manufacturing an optically active alcohol using these enzymes. Modified carbonyl reductases having improved reactivity in the presence of halogen atoms in comparison with the wild type were isolated from a library of modified carbonyl reductases prepared by introducing random mutations into the wild-type enzyme gene. There are also provided enzymes as well as DNA encoding the enzymes, transformants producing the enzymes, and a method of efficiently manufacturing an optically active alcohol using these. |
