Nucleic acid detection and quantification by post-hybridization labeling and universal encoding

摘要

The present invention provides, among other things, methods and compositions for encoding a substrate for detecting and quantifying target nucleic acids.

主权项

1. A kit comprising:
a particle comprising two or more encoding regions and one or more probe regions that are distinct from the encoding regions,
wherein each encoding region is separated from each probe region or encoding region by an inert region,wherein each encoding region bears one or more anchor oligonucleotides,wherein each probe region bears one or more probes of interest; anda plurality of labeled and unlabeled encoding adapters at a ratio to achieve a desired detectable signal level in each encoding region, wherein each individual encoding adapter is designed to specifically bind an individual anchor oligonucleotide and wherein a labeled encoding adapter comprises a detectable moiety;wherein when the particle is incubated with the plurality of labeled and unlabeled encoding adapters under conditions that allow the labeled and unlabeled encoding adapters to bind their corresponding anchor oligonucleotides in the two or more encoding regions, the particle is encoded by generating the desired detectable signal level in the each encoding region.