FUSANTS CAPABLE OF FIXING CARBON OXIDES AND PERFORMING FERMENTATION AND THE PREPARATION METHOD AND USE OF THE SAME

摘要

A microorganism capable of fixing a carbon oxide and performing fermentation and its preparation method and use are provided, wherein the preparation method comprises:
(a) providing a first parental strain which is able to fix a carbon oxide;(b) providing a second parental strain which is able to perform fermentation;(c) preparing a first protoplast and a second protoplast from the first parental strain and the second parental strain, respectively;(d) subjecting the first protoplast and the second protoplast to fuse to provide a fusant; and(e) incubating and selecting the fusant to obtain a target strain.;The target strain is useful in the production of an organic compound such as an organic acid and an alcohol, and one embodiment of the target strain is Clostridium tyrobutyricum ITRI02001, deposited at German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH, DSMZ) under the accession number DSM 32077.

主权项

1. A method for preparing a microorganism capable of fixing a carbon oxide and performing fermentation, comprising:
(a) providing a first parental strain which is able to fix a carbon oxide; (b) providing a second parental strain which is able to perform fermentation; (c) preparing a first protoplast and a second protoplast from the first parental strain and the second parental strain, respectively; (d) subjecting the first protoplast and the second protoplast to fuse to provide a fusant; and (e) incubating and selecting the fusant to obtain a target strain.