COMPOSITIONS AND METHODS FOR INHIBITING HMGB1 ACTIVATION OF MELANOCYTES

摘要

A method of identifying a test agent as a skin tone agent. The method includes determining the level of high-mobility group protein B1 (HMGB1), messenger RNA associated with the expression and/or regulation of HMGB1 (HMGB1 mRNA), and/or micro-RNA associated with the expression and/or regulation of HMGB1 (HMGB1 miRNA) present in the test sample, and identifying the test agent as a skin tone agent when there is no increase in HMGB1 level, a downregulation in transcription of HMGB1 mRNA, and/or an upregulation of HMGB1 miRNA. The method also includes identifying a test agent as a skin tone agent when the test agent inhibits or prevents an increase in melanocyte dendricity and/or body size caused by HMGB1.

主权项

1. A method of identifying a test agent as a skin tone agent, comprising:
a. suspending cells in a first medium at a concentration of between about 1,000 cells/mL and about 2,000,000 cells/mL to provide a test suspension; b. placing at least some of the test suspension in a container; c. contacting at least some of the suspended cells in the container with a test agent to make a test sample; d. determining a level of one or more biomarkers present in the test sample, the one or more biomarkers being selected from high-mobility group protein B1 (HMGB1), messenger RNA associated with the expression and/or regulation of HMGB1 (HMGB1 mRNA), micro-RNA associated with the expression and/or regulation of HMGB1 (HMGB1 miRNA), and combinations of these; e. comparing the level of the biomarker(s) of (d) to a control; and f. identifying the test agent as a skin tone agent when the level of HMGB1 present in the test sample does not correspond to an increase in HMGB1 level, the level of HMGB1 mRNA corresponds to a downregulation in transcription of HMGB1 mRNA, and/or the level of HMGB1 miRNA corresponds to an upregulation of HMGB1 miRNA.