| 发明名称 |
Efficient Deep Sequencing and Rapid Genomic Speciation of RNA Viruses (vRNAseq) |
| 摘要 |
A method for limited input whole genome sequencing of RNA viruses includes isolating a viral RNA sample, converting the viral RNA sample to a double-stranded viral cDNA sample, constructing a double-stranded viral cDNA amplicon library from the double-stranded viral cDNA sample, and sequencing the double-stranded viral cDNA amplicon library to obtain a double-stranded viral cDNA sample sequencing read. |
| 申请公布号 |
US2016076094(A1) |
申请公布日期 |
2016.03.17 |
| 申请号 |
US201514841981 |
申请日期 |
2015.09.01 |
| 申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
Thielen Peter M.;Evans Jared D.;Mehoke Thomas S.;Wolfe Joshua T. |
| 分类号 |
C12Q1/68;C12Q1/70 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for limited input whole genome sequencing of RNA viruses, the method comprising:
isolating a viral RNA sample; converting the viral RNA sample to a double-stranded viral cDNA sample; constructing a double-stranded viral cDNA amplicon library from the double-stranded viral cDNA sample; and sequencing the double-stranded viral cDNA amplicon library to obtain a double-stranded viral cDNA sample sequencing read. |
| 地址 |
BALTIMORE MD US |
