METHOD FOR PRODUCING CEREBELLAR PROGENITOR TISSUE

摘要

The present invention provides a method for producing assemblies of human cells including neural progenitor tissue of the midbrain-hindbrain boundary region by subjecting assemblies of human pluripotent stem cells to suspension culture in serum-free medium containing insulin and treating these assemblies of human pluripotent stem cells or assemblies of human cells derived therefrom by ROCK inhibitor, TGFβ signaling inhibitor, and first fibroblast growth factor in this suspension culture. It is also possible to induce the formation of neuroepithelial structures by neural progenitor cells in this neural progenitor tissue by subjecting assemblies of human cells including neural progenitor tissue of the midbrain-hindbrain boundary region to suspension culture in a serum-free medium and to obtain assemblies of human cells including cerebellar plate tissue.