发明名称 |
DETECTION OF MISFOLDED PROTEINS |
摘要 |
Methods and kits are provided for amplifying and detecting misfolded proteins from samples, for example, from patients having Alzheimer's Disease, Parkinson's Disease, and the like. For example, a method for determining a presence of soluble, misfolded protein in a sample may include contacting the sample with a monomeric, folded protein to form an incubation mixture; conducting an incubation cycle two or more times effective to form an amplified portion of misfolded protein; incubating the incubation mixture effective to cause misfolding and/or aggregation of at least a portion of the monomeric, folded protein; physically disrupting the incubation mixture effective to break up at least a portion of any protein aggregate present; and determining the presence of the soluble, misfolded protein in the sample by detecting at least a portion of the soluble, misfolded protein. The monomeric, folded protein and the soluble, misfolded protein may exclude prion protein (PrP) and isoforms thereof. |
申请公布号 |
WO2016040907(A1) |
申请公布日期 |
2016.03.17 |
申请号 |
WO2015US49844 |
申请日期 |
2015.09.11 |
申请人 |
BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM;AMPRION, INC.;JARA, CLAUDIO SOTO;SHAHNAWAZ, MOHAMMAD;LEBOVITZ, RUSSELL M.;VOLLRATH, BENEDIKT K. |
发明人 |
JARA, CLAUDIO SOTO;SHAHNAWAZ, MOHAMMAD;LEBOVITZ, RUSSELL M.;VOLLRATH, BENEDIKT K. |
分类号 |
C12Q1/00;A61K38/00;G01N33/00 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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