发明名称 |
DETECTION OF MISFOLDED AMYLOID BETA PROTEIN |
摘要 |
Methods and kits are provided for amplifying and detecting Aβ proteins from samples, for example, from patients having Alzheimers Disease. For example, a method for determining a presence of a soluble, misfolded Aβ protein may include contacting the sample with a monomeric, folded Aβ protein to form an incubation mixture; conducting an incubation cycle two or more times on the incubation mixture effective to form an amplified portion of misfolded Aβ protein; incubating the incubation mixture effective to cause misfolding and/or aggregation of at least a portion of the monomeric, folded Aβ protein; physically disrupting the incubation mixture effective to at least partly de-aggregate at least a portion of a misfolded Aβ aggregate present; and determining the presence of the soluble, misfolded Aβ protein in the sample by detecting at least a portion of the amplified portion of misfolded Aβ protein. |
申请公布号 |
WO2016040903(A1) |
申请公布日期 |
2016.03.17 |
申请号 |
WO2015US49840 |
申请日期 |
2015.09.11 |
申请人 |
BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM;AMPRION, INC.;JARA, CLAUDIO SOTO;SHAHNAWAZ, MOHAMMAD;LEBOVITZ, RUSSELL M.;VOLLRATH, BENEDIKT K. |
发明人 |
JARA, CLAUDIO SOTO;SHAHNAWAZ, MOHAMMAD;LEBOVITZ, RUSSELL M.;VOLLRATH, BENEDIKT K. |
分类号 |
G01N33/50;C12Q1/00;G01N33/53 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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