FORMULATION AND METHOD FOR PREPARING SPECIFIC T CELL, AND METHOD FOR PREPARING THE FORMULATION

摘要

The present invention provides a formulation and method for preparing specific T cells, and a method for fabricating the formulation is also disclosed. The formulation can induce specific T cell responses, and comprises at least a cell population of dendritic killer cells presenting specific antigens. In addition, the method mentioned above for preparing specific T cells comprises following steps. A T cell population is provided, and then a formulation of preparing specific T cells is mixing with the T cell population. After cultivating, the specific T cells are harvested. Furthermore, the method for fabricating the above formulation comprises the following steps. First, a cell population of dendritic killer cells is provided. A target sample is then provided, and a step of making the cell population of the dendritic killer cells is co-cultivated with the target sample. After co-cultivating, a cell population of dendritic killer cells presenting specific antigens is harvested.

主权项

1. A method for preparing specific T cells comprising the following steps:
(a) obtaining human peripheral blood mononuclear cells; (b) culturing the cells with IL-15 and 0.5˜20 ng/mL IL-12; (c) sorting the dendritic killer cells, wherein the dendritic killer cells are cells with the cell surface marker HLA-G− CD14− CD19− CD3− CD56+ HLA-DR+; (d) co-culturing the dendritic killer cells with a plurality of target cells, wherein the target cells express a specific antigens; (e) harvesting the dendritic killer cells presenting the specific antigen; (f) mixing the dendritic killer cells presenting the specific antigen with a cell population of T cells and co-culturing for a period; and (g) harvesting the specific T cells.