| 摘要 |
A method for detecting the presence/absence of a fetal chromosomal aneuploidy, said method comprising a first amplification step for amplifying chromosomal DNA obtained from a biological sample that is collected from a pregnant woman to give a first amplification product, a second amplification step for multiplex amplifying a plurality of target regions with the use of the first amplification product as a template to give a second amplification product, a step for labeling both ends of the second amplification product to give a labeled second amplification product, a third amplification step for using a primer pair annealing to the labels and performing amplification with the use of the labeled second amplification product as a template to give a third amplification product, and a step for determining the base sequence and amplification amount of the target regions from the third amplification product, wherein the amplification in the first amplification step is 6,000- to 30,000-fold in terms of the total DNA amount, and the amplification in the second amplification step is 3- to 150-fold in terms of the total DNA amount. |
