发明名称 |
COMPOSITIONS AND METHODS FOR THE EXPRESSION OF CRISPR GUIDE RNAS USING THE H1 PROMOTER |
摘要 |
The presently disclosed subject matter provides compositions and methods for the expression of CRISPR guide RNAs using the H1 promoter. In particular, compositions and methods are provided for the use of the H1 promoter to express CRISPR guide RNA (gRNA) with altered specificity of the 5′ nucleotide, as well as use of the H1 promoter sequence as a bidirectional promoter to express Cas9 nuclease and the gRNA simultaneously. Compositions and methods are also provided for the expression and regulation of gRNA expression in vivo through the use of RNA ribozymes and regulatable aptazymes. |
申请公布号 |
US2016074535(A1) |
申请公布日期 |
2016.03.17 |
申请号 |
US201514951240 |
申请日期 |
2015.11.24 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
RANGANATHAN VINOD;ZACK DONALD |
分类号 |
A61K48/00;C12N15/86 |
主分类号 |
A61K48/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Baltimore MD US |