| 摘要 |
The present invention provides: a split-type recombinant protein; a gene encoding the split-type recombinant protein; and an analysis method using the split-type recombinant protein or the gene. The split-type recombinant protein comprises: an N-terminal-sided fragment of firefly luciferase, which is one of two split fragments of firefly luciferase that is split into two fragments so as to recover the firefly luciferase activity thereof when the two fragments are linked to each other; a C-terminal-sided fragment of firefly luciferase, which is the other of the two split fragments and also carries 58 to 78 amino acid residues that lie from a splitting position, at which the firefly luciferase can be split into the two fragments so as to recover the firefly luciferase activity thereof when the two fragments are linked to each other, toward the N-terminal of the firefly luciferase; and a linker peptide. In the split-type recombinant protein, the firefly luciferase activity can be recovered when the N-terminal-sided fragment and the C-terminal-sided fragment are linked to each other. |
