METHODS FOR DETECTING AND QUANTIFYING VIABLE BACTERIAL ENDO-SPORES

摘要

Methods and systems for detecting viable bacterial endospores in a sample and related methods to quantify viable bacterial endospores in a sample.

主权项

1. A method for detecting a viable bacterial endospore in a sample, the method comprising
contacting the sample with an agent capable of labeling a first form of nucleic acid to produce a labeled first form of nucleic acid, the agent further capable to interfere with amplification of the labeled first form of nucleic acid, the first form of nucleic acid consisting of a nucleic acid not comprised in the viable endospore; treating the sample following the contacting to produce an extract, the treating performed for a time and under conditions to allow extraction from the sample of a nucleic acid comprising the first form of nucleic acid and a second form of nucleic acid, the second form of nucleic acid consisting of a nucleic acid comprised in the viable bacterial endospore; performing nucleic acid amplification of the extract; detecting an amplification product in the extract following nucleic acid amplification to detect viable endospores in the sample.