| 摘要 |
The present invention relates to trifunctional crosslinking reagents as defined in claim 1, where HR N is a hydrazine-protecting group selected from hydrazones (R'R"C=NNH 2 ), said hydrazone being aldehyde or ketone hydrazone, said substituents R', R" being selected from hydrogen, substituted (C1-C6)alkyl, substituted aryl and substituted heteroaryl. The invention further provides trifunctional crosslinking reagents suitable for the detection, isolation and purification of captured glycopeptides, their methods of production, as well as their use in methods for detecting, identifying and characterizing interactions between ligands and their corresponding glycoprotein target receptors on living cells and in biological fluids. |
