Method for preparing mutant <i>Escherichia coli </i>capable of simultaneously utilizing glucose and xylose

摘要

The present invention relates to a method for preparing a mutant E. coli strain, capable of simultaneously using glucose and xylose, by genetic engineering and evolutionary adaptation; the mutant E. coli prepared using the same; and a method for producing biofuels, biologically active ingredients, medicinal materials or base chemicals for the chemical industry using the mutant E. coli. Being capable of simultaneously using glucose and xylose, in contrast to wild-type E. coli, the mutant E. coli can be effectively applied to the enzymatic saccharification process of producing biofuels from a biomass.

主权项

1. A method for preparing a mutant E. coli capable of simultaneously utilizing glucose and xylose from a wild-type E. coli, comprising:
(1) replacing inducible promoters of araBAD operon, araFGH operon, araE gene, xylAB operon, and xylFGH operon of the wild-type E. coli with respective constitutive promoters; and (2) growing the promoter-replaced E. coli in a xylose minimal medium or an arabinose and xylose minimal medium for 10 days or longer.