HIGH-CELL DENSITY FED-BATCH FERMENTATION PROCESS FOR PRODUCING RECOMBINANT PROTEIN

摘要

Disclosed are methods for producing proteins, for example, recombinant meningococcal 2086 proteins. Embodiments include using fed-batch fermentation with continuous input of an inducer. Also disclosed are methods for large scale production of a recombinant meningococcal protein in a recombinant bacterial cell culture, comprising: continuously adding a carbon source to the culture and continuously adding an inducer to the culture after the culture achieves a threshold parameter, said inducer being added simultaneously to the carbon source; and isolating the meningococcal protein from the culture; wherein the meningococcal protein is produced at a peak density after the inducer is added to the culture for about 3 hours; wherein the maximum total amount of the inducer added to the culture is about 20 g/L; wherein the inducer is arabinose and the feed rate is between 1.7 and 6.7 g/L/h; and wherein the carbon source feed rate is between 2.25 and 7.5 g/L/h.