Axial illumination for capillary electrophoresis

摘要

System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light. The system for analyzing samples comprising: a light source that provides a non-coherent excitation light; at least one housing, wherein the housing transports samples and propagates the non-coherent excitation light by total internal reflection; a coupling optical element configured to introduce the non-coherent excitation light into the at least one housing through a wall of the at least one housing; and at least one NA enhancing optical element to collect an emitted fluorescence, wherein the NA enhancing optical element is constructed of a first material and the housing is constructed of a second material, wherein the first material has a greater index of refraction than the second material.

主权项

1. A system for analyzing a biological sample, comprising:
a light source configured to provide excitation light; a housing configured to contain a biological sample and to receive the excitation light from the light source, the housing comprising a material having a first index of refraction; at least one NA enhancing optical element comprising a material having a second index of refraction that is greater than the first refractive index, the NA enhancing optical element configured to receive and pass an emission of fluorescent light from the biological sample when the biological sample is located within the housing; and a mask comprising at least one aperture, the mask configured to receive and pass the emission from the biological sample and from the NA enhancing aperture when the biological sample is located within the housing.