| 发明名称 | Methods of identifying a cellular nascent RNA transcript | ||
| 摘要 | Methods and compositions for identifying a cellular nascent RNA transcript are provided. | ||
| 申请公布号 | US9284604(B2) | 申请公布日期 | 2016.03.15 |
| 申请号 | US201313900356 | 申请日期 | 2013.05.22 |
| 申请人 | The Regents of the University of California | 发明人 | Weissman Jonathan;Churchman Lee Stirling |
| 分类号 | C12Q1/68;C07H21/00 | 主分类号 | C12Q1/68 |
| 代理机构 | Mintz, Levin, Cohn, Ferris, Glovsky and Popeo, P.C. | 代理人 | Mintz, Levin, Cohn, Ferris, Glovsky and Popeo, P.C. |
| 主权项 | 1. A method of identifying a cellular nascent RNA transcript, comprising: (a) arresting transcription in a cell; (b) purifying a native cellular nascent RNA transcript; and (c) sequencing said native cellular nascent RNA transcript thereby identifying the cellular nascent RNA transcript; wherein said native cellular nascent RNA transcript is purified as part of an RNA polymerase complex, wherein said cellular nascent RNA transcript does not comprise a labeled nucleotide and wherein said cellular nascent RNA transcript is not crosslinked to an RNA polymerase complex. | ||
| 地址 | Oakland CA US | ||