Targeted analysis for tandem mass spectrometry

摘要

A tandem mass spectrometer and method are described. Precursor ions are generated in an ion source and an ion injector injects ions towards a downstream ion guide via a single or multi reflection TOF device that separates ions into packets in accordance with their m/z. A single pass ion page in the path of the precursor ions between the ion injector and the ion guide is controlled so that only a subset of precursor ion packets, containing precursor ions of interest, is allowed onward transmission to the ion guide. A high resolution mass spectrometer is provided for analysis of those ions, or their fragments, which have been allowed passage through the ion gate. The technique permits multiple m/z ranges to be selected from a wise mass range of precursors, with optional fragmentation of one or more of the chosen ion species.

主权项

1. A method of tandem mass spectrometry, comprising the steps of:
a) generating ions in an ion source; b) guiding the ions into an ion injector and ejecting an ion beam from the ion injector; c) temporally separating a plurality of ion packets of the ejected ion beam, each packet containing ions of a respective one of a plurality of different ion species; d) sequentially selecting from the plurality of ion packets a subset of a plurality of ion packets; e) mixing the selected subset of a plurality of ion packets; and f) analyzing the resulting ion population derived from the mixed selected subset of ion packets in a high resolution mass analyzer, wherein the transmission of ion packets of the selected subset is based on their relative intensities in the ion beam.