DNA polymerases with increased 3′-mismatch discrimination

摘要

Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

主权项

1. A recombinant nucleic acid encoding a DNA polymerase comprising a motif in the polymerase domain comprising K-L-K-X2T-X3-X4-D-P-L-P, wherein: X2 is S or N; X3 is an amino acid other than Y; and X4 is V or I (SEQ ID NO:10); wherein the DNA polymerase has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:1.