发明名称 |
METHOD FOR HARVESTING CULTURE PRODUCT |
摘要 |
The present invention provides a more productive method for culturing and a more productive method for harvesting culture product in cell culture wherein the cell produces the culture product. The present invention relates to a method for harvesting a culture product contained in a culture solution in the cell culture wherein the cell produces the culture product, comprising the following steps: B sending the culture solution to a filtration membrane; C: filtering the culture solution by alternating tangential flow filtration while changing the flow of the culture solution so as to cause a reciprocating motion thereof in a direction parallel with the surface of the filtration membrane to obtain a filtrate; D: sending back a culture solution residue that has remained without permeating the filtration membrane; and G: harvesting the culture product from the filtrate, wherein
the filtration membrane used in the step B is a porous membrane having an average pore size of 20 μm or larger and 100 μm or smaller for the pores in the surface of the culture solution side or a porous membrane in which the ratio of pores having a diameter smaller than 20 μm for the pores in the surface of the culture solution side is 50% or less of all pores in the surface of the culture solution side. |
申请公布号 |
US2016068565(A1) |
申请公布日期 |
2016.03.10 |
申请号 |
US201514640511 |
申请日期 |
2015.03.06 |
申请人 |
ASAHI KASEI MEDICAL CO., LTD. |
发明人 |
SHIBANO Masafumi;KATO Chihiro |
分类号 |
C07K1/34;B01D63/02;C07K16/00;B01D71/58;B01D71/68;B01D61/14;B01D69/02 |
主分类号 |
C07K1/34 |
代理机构 |
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代理人 |
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主权项 |
1. A method for harvesting a culture product contained in a culture solution in cell culture wherein the cell produces the culture product, comprising following steps:
B: sending the culture solution to a filtration membrane; C: filtering the culture solution by alternating tangential flow filtration while changing the flow of the culture solution so as to cause a reciprocating motion thereof in a direction parallel with the surface of the filtration membrane to obtain a filtrate; D: sending back a culture solution residue that has remained without permeating the filtration membrane; and G: harvesting the culture product from the filtrate, wherein the filtration membrane used in the step B is; a porous membrane having an average pore size of 20 μm or larger and 100 μm or smaller for the pores in the surface of the culture solution side: or a porous membrane in which the ratio of pores having a diameter smaller than 20 μm for the pores in the surface of the culture solution side is 50% or less of all pores in the surface of the culture solution side. |
地址 |
Tokyo JP |