Plant genome modification using guide RNA/Cas endonuclease systems and methods of use
摘要
Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
申请公布号
AU2014308896(A1)
申请公布日期
2016.03.10
申请号
AU20140308896
申请日期
2014.08.20
申请人
E. I. DU PONT DE NEMOURS AND COMPANY;PIONEER HI-BRED INTERNATIONAL, INC.
发明人
CIGAN, ANDREW;FALCO, SAVERIO CARL;GAO, HUIRONG;LI, ZHONGSEN;LIU, ZHAN-BIN;LYZNIK, L. ALEKSANDER;SHI, JINRUI;SVITASHEV, SERGEI;YOUNG, JOSHUA K.