摘要 |
This invention encompasses methods of making 1 -propanol. In some embodiments the methods comprise providing a cultured bacterial biofilm; culturing the bacterial biofilm under conditions suitable for production of 1 -propanol; and collecting 1 - propanol produced by the biofilm culture. In some embodiments the methods comprise providing a bacterial culture comprising bacteria and culture media, wherein the culture media comprises a concentration of threonine higher than that present in LB; maintaining the bacterial culture under conditions suitable for production of 1 -propanol; and collecting 1 -propanol produced by the culture. This invention also encompasses bacterial culture systems. In some embodiments the bacterial culture systems comprise a bacterial biofilm comprising bacteria growing on an artificial solid substrate; culture media; 1 - propanol in liquid and/or gas form; and a collection device configured to collect 1 - propanol produced by the culture. In come embodiments the culture systems comprise bacteria; culture media, wherein the culture media comprises a concentration of threonine higher than that present in LB; 1 -propanol in liquid and/or gas form; and a collection device configured to collect 1-propanol produced by the culture. |