NOVEL RECOMBINANT BACULOVIRUS VECTOR AND USES THEREOF

摘要

Disclosed herein is a recombinant viral construct and its uses thereof. The recombinant viral construct is capable of simultaneously expressing three exogenous proteins, including a classical swine fever virus (CSFV) antigen, a porcine circovirus type 2 (PCV2) antigen, and an immunomodulatory protein. The recombinant viral construct is hence useful as a bio-tool for simultaneously producing multiple antigens of a bi-subunit vaccine.

主权项

1. A recombinant viral construct for producing a bi-subunit vaccine, comprising,
a promoter; a first expression cassette, operably linked to the promoter, wherein the first expression cassette comprises in sequence, a first nucleic acid encoding a classical swine fever virus (CSFV) antigen, a self-cleaving element encoding a self-cleaving peptide, and a second nucleic acid encoding a porcine circovirus type 2 (PCV2) antigen; a second expression cassette, operably linked to the promoter, er, wherein the second expression cassette comprises in sequence, a first internal ribosomal entry site (IRES) element, and a third nucleic acid encoding a reporter polypeptide; and a third expression cassette, operably linked to the promoter, wherein the third expression cassette comprises in sequence, a second IRES element, and a fourth nucleic acid encoding an immunomodulatory polypeptide; wherein the CSFV antigen is CSFV-E2 and has an amino acid sequence or SEQ ID No: 1; and the PCV2 antigen is PCV2 open reading frame 2 (ORF-2) and has an amino acid sequence of SEQ ID NO: 2.