发明名称 |
MICROSCOPE AND METHOD FOR HIGH-RESOLUTION 3-D FLUORESCENT MICROSCOPY |
摘要 |
In a sample, fluorescence emitters are repeatedly excited to emit fluorescence, and still images are produced of the sample by means of a microscope. At least a subset of the fluorescence emitters is isolated in each still image. The positions of the fluorescence emitters are localized in the still images with a location accuracy exceeding the optical resolution. A high-resolution composite image is generated therefrom. An adaptive mirror is arranged in the imaging beam path, and is adjusted in such a manner that it produces an astigmatism when at least one of the still images is produced. As a result, still images with astigmatism are captured. Depth position information for the fluorescence emitters is derived from the rotational asymmetry. The adaptive mirror is additionally adjusted in such a manner that it does not produce any astigmatism when some of the still images are produced. |
申请公布号 |
EP2802921(B1) |
申请公布日期 |
2016.03.09 |
申请号 |
EP20120806018 |
申请日期 |
2012.12.13 |
申请人 |
CARL ZEISS MICROSCOPY GMBH |
发明人 |
KALKBRENNER, THOMAS;WOLLESCHENSKY, RALF |
分类号 |
G02B21/16;G01N21/64;G02B21/36;G02B27/58 |
主分类号 |
G02B21/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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