A MUTANT OF METHYLOSINUS TRICHOSPORIUM OB-3B SMMO HYDROXYLASE IMPROVED IN ACTIVITY AND USE OF THE SAME

摘要

The purpose of the present invention is to increase enzymatic activities and the stability of sMMO hydroxylase derived from Methylosinus trichosporium OB-3b by using a partial mutation technique and a technique of quantum mechanics and molecular dynamics. More specifically, the present invention relates to: sMMO hydroxylase with improved enzymatic activies by the searched residue of a mutant, for finding the residue which influences the enzymatic stability through screening on the basis of quantum mechanics and molecular dynamics; a nucleic acid molecule for coding the same; a vector comprising the nucleic acid molecule; a transformant comprising the vector; a mutant of the sMMO hydroxylase; and a manufacturing method of the improved sMMO hydroxylase.