A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.
申请公布号
EP2992104(A1)
申请公布日期
2016.03.09
申请号
EP20140721474
申请日期
2014.04.29
申请人
FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LIMITED
发明人
SAUNDERS, FAY LOUISE;DODDS, ANNA LOUISE;BAYARD, ADELINE MARIE GERALDINE;KARA, BHUPENDRA VALLABH